Concentration of different roadside soil amendment (0, 50, 75 and 100%) were
prepared by mixing with garden (control) soil. All pollution parameters were
determined in the different concentrations of soil i.e, pH, TDS, ECe, CO3, HCO3. The
values of all these parameters increased with increased the concentrations of soil and
increasing trend was Pb>Cd. Nerium indicum L. was grown in these concentrations for
35 days of the experiment. The growth parameters i.e, root length, shoot length, no. of
roots and no. of leaves were recorded in all soil concentrations. The maximum growth
was found to be in 100% and minimum in 50% and no growth was observed in 0%
concentrations. The accumulation of heavy metals Pb and Cd was observed and
increasing trend was Pb>Cd. The amount of protein was also detected in all plants that
grown in different concentrations of soil. The amount of protein increased with
increasing the concentrations. The SOD was determined in plants parts increased with
increasing concentrations of soil. The aim of the study was to check the effect of heavy
metals in Nerium indicum L. by growing in different concentrations of roadside soil.
The plant was further assessed to determine the role of protein and antioxidants
activities.