A study was carried out to extract the oil from spent bleaching earth (SBE), and physico-chemical properties and fatty acids composition were compared with unrefined and refined canola oil. Oil was extracted from SBE through solvent extraction using n-hexane and oil with 30.12% yield was obtained. Fatty acids constituents were determined by gas chromatography-flame ionization detector (GC-FID) after esterification of the oil with methanol in the presence of boron trifluoride. Major constituents were found to be 67.87% stearic acid and 12.8488% linoleic acid in oil extracted from SBE, 39.89% oleic acid and 12.5963% in crude canola oil and 12.089% linolenic acid in refined oil. Maximum values of FFA (56.74%), acid value (112.91mg KOH/g), peroxide value (11.65 mEq/kg), moisture content (2.77%) and colour (yellow=10, red=30) were found in oil extracted from SBE and maximum iodine value (104), saponification value (195.17mg KOH/g) and refractive index (1.4664) were found in refined oil.