000			02435nam a22001697a 4500
999			_c21870 _d21870
005			20221018164512.0
008			221018b ||||| |||| 00| 0 eng d
020			_ahbk
082			_a591 _bId26
100			_aSana Akbar, _bBS Zoology, _c2015-2019, _dSupervised by Dr. Afia M. Akram
245			_aIndentification of C-kit exon 11 mutations and prognostic imortance in acute myeloid leukemia (AML) patients from Pakistan _c/ Sana Akbar
260			_aLahore : _bDivision of Science & Technology, University of Education, _c2019
300			_axiii, 58 p. _eCD
520			_aLeukemia is designated as abnormal division of blood cells. Blood cells begin to proliferate abnormally and fail to differentiate into normal cells. Acute myeloid leukemia is a heterogeneous disorder characterized by abnormal proliferation and immature production of hematopoietic stem cells. Major causes of AML might be mutations, translocations or any chromosomal abnormality. C-kit is a proto oncogene which is related to Receptor Tyrosine Kinase family and have a significant influence on cell proliferation, maturation and survival. Exon 11 of c-kit gene is sequenced and analyzed to study its prognosis and role in AML among Pakistani patients. Aim of this study are to focus on hematological (blast cells percentage, TLCs, hemoglobin level etc.) and clinical parameters (hepatomegaly, splenomegaly, FAB classification etc.) associated with mutation in exon 11 of c-kit gene and its prognosis. Mutations in exon 11 of c-kit gene are studied by polymerase chain reaction (PCR) and Sanger sequencing technique in current investigations. All sequences are studied by genius prime 2019 and statistically analyzed by Chi square Fischer’s exact test. Two out of 31 patients of AML are observed having mutation in this exon at codon 555. Both patients have same mutation resulted glutamic acid in place of valine. Mutations in exon 11 of c-kit gene are only found associated with cytogenetic, statistically. However all other parameters are not significantly associated with this exon and have p value higher than 0.05. Prognostic implications of c-kit are still unclear. However, mutations RTK have poor influence on outcome. These findings helped us to study prognosis of c-kit and its association with hematological and clinical parameters among AML patients.
650			_aZoology--Acute Myeloid--Leukemia--Patients--Pakistan
942			_cTH