000			03293nam a22001577a 4500
999			_c21663 _d21663
005			20221021141753.0
008			220801b ||||| |||| 00| 0 eng d
082			_a591 _bIs71
100			_aAyesha Idrees, _bMPhil(Zoology) _c2016-2018 _dSupervised by Dr.Shagufta Andleeb
245			_aIsolation and Characterization of a Therapeutic Bacteriophage Against Pseudomonas Sp. _c/Ayesha Idrees
260			_aLahore : _bDepartment of Zoology, Div. S&T University of Education, _c2018
300			_a80 p. _bxiv _eCD
520			_aAntibiotics are the drugs or medicines that are obtained from livings organisms and used to treat bacterial or fungal infections. Long term use of antibiotics against a certain microorganism lead to the adaptation of microorganism against that specific antibiotic which is commonly called as antibiotic resistance. Antimicrobial resistance is the capability of that organism that can be seen under the microscope. It is their prime quality that it opposes the impact of an antitoxin. Bacteriophages are infectious agents which attack on bacteria cells, on lytic phages, disturb bacteria digestion, and destruct them. Lytic Bacteriophages are used as phage therapy to cure pathogenic maladies. The Research aim was to characterize the bacteriophage specific to bacterium Pseudomonas aeruginosa and to study bacteriostatic efficiency (reduction capacity) of phages specific to the PA-1. For characterization of bacteriophage IQL against clinically isolated Pseudomonas aeruginosa strain sample was collected from different areas of Lahore but the required sample was finalized from Iqbal Town, Lahore. The sample was processed for bacteriophage enrichment by standard protocols. Then, Bacteriophage was characterized by temperature stability, pH stability, storage stability and reduction assay to reduce the bacterial growth and one step growth curve to calculate the burst time and then its genome was characterized. The Bacteriophage IQL has a narrow spectrum host range. It was effective against 20/38 P. aeruginosa, 1/3 Klebsiella pneumoniae, ½ S. marcescens strains and have no lytic activity against Acinetobacter baumannii, Escherichia coli, Burkholdera cepacia, and enterobacter strains under study. The titer of the Bacteriophage IQP was counted 9.4×1021 PFUs/ml. Bacteriophage IQL was stable at pHysiological pH range of 5-9. The best activity is observed at alkaline pH of 9, whereas it showed slight decrease in the PFUs at pH below 9. For survey of temperature impacts on bacteriophage suitability, different temperatures were used to ascertain titer for one hour phages cure. The bacteriophage stabled on 25, 37 and 45 oC. After curing at 60 oC on phages noticeable decrease seen in titer. On 4 oC, 25 oC, and 37 oC temperature titer reduction was not seen following storage of one month in phage IQL. Slight decrease in the titer was seen at -20 and - 80oC for one month. IQL 2 bacteriophage reduces the bacterial growth completely till 12 hours at MOI 10. One stage growth curve for the phage IQL was done. The burst time and the burst estimate were figured. Keywords: Pseudomonas aeruginosa, Bacteriophage, Genome characterization of bacteriophage
650			_aIsolation - Characterization - Bacteriophage - Zoology
942			_cTH