000 01321nam a22001817a 4500
999 _c19554
_d19554
005 20200818123826.0
008 200131b ||||| |||| 00| 0 eng d
020 _a9788173715785 (pbk)
040 _cPK-IsLIS
082 _a591
_bG1546
100 _aGangal, Sudha
245 _aPrinciples and practice of animal tissue culture
_c/ Sudha Gangal
260 _aHyderabad :
_bUniversities Press,
_c2007
300 _a230 p.
650 _aZoology
942 _cBK
505 0 _a1. Designing the tissue culture laboratory 2. Equipping the tissue culture laboratory 3. Glassware and plasticware used for tissue culture 4. Aseptic techniques 5. Sterilisation of materials to be used for tissue culture 6. Tissue culture media 7. Types of tissue culture 8. Organ and organotypic cultures 9. Primary cultures 10. Transformation, differentiation and dedifferentiation 11. Culture of cell lines 12. Protocols for routine characterization of cell lines 13. Specialized techniques in tissue culture 14. Stem cells cultures 15. Autoradiography of cultured cells 16. Flow cytometric analysis of cultured cells 17. Flow cytometric analysis of lymphocytes and cultured cells 18. Hybridoma cultures to generate monoclonal antibodies 19. Laser confocal microscopy and its application in cell biology 20. Troubleshooting