000			01417nam a22001937a 4500
999			_c11533 _d11533
005			20200714131923.0
008			181031b ||||| |||| 00| 0 eng d
040			_cPK-IsLIS
082			_a572.8 _bW1511
100			_aWalker, M. John
245			_aMethods in molecular biology, basic protein and peptide protocols _c/ John M. Walker
260			_aIslamabad : _bNBF,
300			_axii, 490 p.
490			_v32
650			_aEducation
650			_aBiology
942			_cBK
505	0		_aPreface; Contributors; the lowry method for protein quantitation; the bicinchoninic acid assay for protein quantitation; the braford method for protein quantitation; nondenaturing Polyacrylamide gel electriohoresis of Proteins; SDS Polyacrylamide gel electriohoresis of Proteins; gradient SDS Polyacrylamide gel electriohoresis of Proteins; Acetic acid-urea Polyacrylamide gel electriohoresis of Proteins; Electrophoresis of peptides; Isoelectric focusing of proteins in Ultra-thin Polyacrylamide gels; The In vivo isotopic labeling of proteins for polyacrylamide gel eltrophoresists; Structural Profinlings of OLigosaccharides of Glycoproteins; Drying gels; Comparison of Protein Primary Structures: peptide Mapping; Amino Acid Analysis; Enzymatic methods for cleaving protein; Chemical Modification of proteins; The raising of a polyclonal antiserum to a protein; Enzyme-linked Immunosorbent assay