Sana Akbar,  BS Zoology,  2015-2019,  Supervised by Dr. Afia M. Akram <br/><br/>
Indentification of C-kit exon 11 mutations and prognostic imortance in acute myeloid leukemia (AML) patients from Pakistan 
/ Sana Akbar 
 - Lahore :  Division of Science & Technology, University of Education,   2019 
 - xiii, 58 p.   CD 
<br/><br/> Leukemia is designated as abnormal division of blood cells. Blood cells begin to proliferate<br/>abnormally and fail to differentiate into normal cells. Acute myeloid leukemia is a<br/>heterogeneous disorder characterized by abnormal proliferation and immature production of<br/>hematopoietic stem cells. Major causes of AML might be mutations, translocations or any<br/>chromosomal abnormality. C-kit is a proto oncogene which is related to Receptor Tyrosine<br/>Kinase family and have a significant influence on cell proliferation, maturation and survival.<br/>Exon 11 of c-kit gene is sequenced and analyzed to study its prognosis and role in AML among<br/>Pakistani patients. Aim of this study are to focus on hematological (blast cells percentage,<br/>TLCs, hemoglobin level etc.) and clinical parameters (hepatomegaly, splenomegaly, FAB<br/>classification etc.) associated with mutation in exon 11 of c-kit gene and its prognosis.<br/>Mutations in exon 11 of c-kit gene are studied by polymerase chain reaction (PCR) and Sanger<br/>sequencing technique in current investigations. All sequences are studied by genius prime 2019<br/>and statistically analyzed by Chi square Fischer’s exact test. Two out of 31 patients of AML<br/>are observed having mutation in this exon at codon 555. Both patients have same mutation<br/>resulted glutamic acid in place of valine. Mutations in exon 11 of c-kit gene are only found<br/>associated with cytogenetic, statistically. However all other parameters are not significantly<br/>associated with this exon and have p value higher than 0.05. Prognostic implications of c-kit<br/>are still unclear. However, mutations RTK have poor influence on outcome. These findings<br/>helped us to study prognosis of c-kit and its association with hematological and clinical<br/>parameters among AML patients. 
<br/><br/><label>ISBN: </label>hbk 
<br/><br/><label>Subjects--Topical Terms: </label><br/>Zoology--Acute Myeloid--Leukemia--Patients--Pakistan
<br/><br/><label>Dewey Class. No.: </label>591  / Id26