Methods in molecular biology, basic protein and peptide protocols
/ John M. Walker
- Islamabad : NBF,
- xii, 490 p.
- 32 .
Preface; Contributors; the lowry method for protein quantitation; the bicinchoninic acid assay for protein quantitation; the braford method for protein quantitation; nondenaturing Polyacrylamide gel electriohoresis of Proteins; SDS Polyacrylamide gel electriohoresis of Proteins; gradient SDS Polyacrylamide gel electriohoresis of Proteins; Acetic acid-urea Polyacrylamide gel electriohoresis of Proteins; Electrophoresis of peptides; Isoelectric focusing of proteins in Ultra-thin Polyacrylamide gels; The In vivo isotopic labeling of proteins for polyacrylamide gel eltrophoresists; Structural Profinlings of OLigosaccharides of Glycoproteins; Drying gels; Comparison of Protein Primary Structures: peptide Mapping; Amino Acid Analysis; Enzymatic methods for cleaving protein; Chemical Modification of proteins; The raising of a polyclonal antiserum to a protein; Enzyme-linked Immunosorbent assay