The Schiff base ligand and its metal complexes were designed and collected from
Chemistry department as potential antimicrobial agent and structure activity relationship
could be established following in vitro assays against human pathogens. The broth
dilution method was used to determine percent antibiosis. Testing compounds were
diluted upto 1.5mg/ml. Ciprofloxacin was used as a positive control. Most of the
chemicals inhibited the growth of all pathogens but percentage of antibiosis varied for
different pathogens. The Schiff bases and their derivatives exhibited percent antibiosis
against Escherichia coli (23.67-99.15), Pseudomonas aeruginosa (32.36-99.49),
Staphylococcus aureus (33.19-99.26) and klebsiella sp. (20.93-99.51). The
Bis((N1E,N2E)-N1,N2-bis(4-methoxybenzylidene)-benzene-1,2-diamine)plumbum (II)acetate
(MS-89) and Bis(3-(1-(4 hydroxyphenylimino)ethyl)dihydrofuran-2(3H)-one nickel(II)nitrate
(AK-25) showed better antibiotic potential (›90%) against two pathogens i.e; E. coli and
Klebsiella sp. But Bis(3-(1-(4 hydroxyphenylimino)ethyl)dihydrofuran-2(3H)-one
nickel(II)nitrate (AK-25) showed no activity against P. aeruginosa. Yet, the (E)-N-(2-
nitrobenzylidene) 3-nitroaniline (AH-4B) exhibited highest percent antibiosis (›90%) with
two pathogens (P. aeruginosa and S. aureus). The 3-(1-(4-
hydroxyphenylimino)ethyl)dihydrofuran-2(3H)-one (AK-22) inhibited four pathogens in
range of 68-83%. The two pathogens i.e; P. aeruginosa and S. aureus developed
resistance against Bis(3-(1-(2-(2,4dinitrophenyl)hydrazono)ethyl)dihydrofuran-2(3H)-
one)cobalt(II)chloride (AK-19). The compounds (MS-89, AH-4B) with better antibiosis
against four human pathogens can be used as chemotherapeutic agent in pharmaceutical
industry ton treat pathogenic infectious diseases.
Key Word: Schiff base, P. aeruginosa, S. aureus, Klebsiella, E.coli, Antimicrobial
activity, Percent inhibition, Bacterial potential.