Indentification of C-kit exon 11 mutations and prognostic imortance in acute myeloid leukemia (AML) patients from Pakistan (Record no. 21870)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02435nam a22001697a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20221018164512.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 221018b ||||| |||| 00| 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | hbk |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 591 |
| Item number | Id26 |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Student name | Sana Akbar, |
| Class | BS Zoology, |
| Session | 2015-2019, |
| Supervisor | Supervised by Dr. Afia M. Akram |
| 245 ## - TITLE STATEMENT | |
| Title | Indentification of C-kit exon 11 mutations and prognostic imortance in acute myeloid leukemia (AML) patients from Pakistan |
| Statement of responsibility, etc | / Sana Akbar |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Lahore : |
| Name of publisher, distributor, etc | Division of Science & Technology, University of Education, |
| Date of publication, distribution, etc | 2019 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xiii, 58 p. |
| Accompanying material | CD |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Leukemia is designated as abnormal division of blood cells. Blood cells begin to proliferate<br/>abnormally and fail to differentiate into normal cells. Acute myeloid leukemia is a<br/>heterogeneous disorder characterized by abnormal proliferation and immature production of<br/>hematopoietic stem cells. Major causes of AML might be mutations, translocations or any<br/>chromosomal abnormality. C-kit is a proto oncogene which is related to Receptor Tyrosine<br/>Kinase family and have a significant influence on cell proliferation, maturation and survival.<br/>Exon 11 of c-kit gene is sequenced and analyzed to study its prognosis and role in AML among<br/>Pakistani patients. Aim of this study are to focus on hematological (blast cells percentage,<br/>TLCs, hemoglobin level etc.) and clinical parameters (hepatomegaly, splenomegaly, FAB<br/>classification etc.) associated with mutation in exon 11 of c-kit gene and its prognosis.<br/>Mutations in exon 11 of c-kit gene are studied by polymerase chain reaction (PCR) and Sanger<br/>sequencing technique in current investigations. All sequences are studied by genius prime 2019<br/>and statistically analyzed by Chi square Fischer’s exact test. Two out of 31 patients of AML<br/>are observed having mutation in this exon at codon 555. Both patients have same mutation<br/>resulted glutamic acid in place of valine. Mutations in exon 11 of c-kit gene are only found<br/>associated with cytogenetic, statistically. However all other parameters are not significantly<br/>associated with this exon and have p value higher than 0.05. Prognostic implications of c-kit<br/>are still unclear. However, mutations RTK have poor influence on outcome. These findings<br/>helped us to study prognosis of c-kit and its association with hematological and clinical<br/>parameters among AML patients. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Zoology--Acute Myeloid--Leukemia--Patients--Pakistan |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Theses |
| Withdrawn status | Damaged status | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| UE-Central Library | UE-Central Library | 18.10.2022 | 591 Id26 | TTH180 | 18.10.2022 | 18.10.2022 | Theses |
