Plant molecular biology manual / (Record no. 18950)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03681nam a22002177a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20200616093747.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 190715b ||||| |||| 00| 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9789401176545 (pbk) |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | PK-IsLIS |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 581.35 |
| Item number | St262 |
| 245 ## - TITLE STATEMENT | |
| Title | Plant molecular biology manual / |
| Statement of responsibility, etc | edited by Stanton B. Gelvin, Robbert A. Schilperoort |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 2nd ed. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher, distributor, etc | Springer Science, |
| Date of publication, distribution, etc | 1994 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | various pages |
| 546 ## - LANGUAGE NOTE | |
| Language note | Eng. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Plant physiology |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Molecular biology |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Plant molecular biology |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Books |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Section A : In vitro methods of gene transfer to plant cells.- 1. PEG-mediated direct gene transfer and electroporation.- 2. Gene transfer to plants via particle bombardment.- Section B: Agrobacterium-mediated gene transfer to plant cells.- 1. Agrobacterium-mediated gene transfer to plant cells: cointegrate and binary vector systems.- 2. Specialized vectors for gene tagging and expression studies.- 3. Agrobacterium molecular genetics.- 4. Genetic manipulation of Agrobacterium tumefaciens strains to improve transformation of recalcitrant plant species.- 5. Transient expression assays using GUS constructs and fluorometric detection for analysis of T-DNA transfer.- 6. Agrobacterium inoculation techniques for plant tissues.- Section C: Selectable and screenable markers for plant transformation.- 1. Antibiotic-resistance markers for plant transformation.- 2. Reporter genes for plants.- 3. Opines as screenable markers for plant transformation.- Section D: Nucleic acid extraction from plant tissue.- 1. Extraction of total cellular DNA from plants, algae and fungi.- 2. Isolation and characterization of nuclear scaffolds.- 3. Isolation of plant mitochondria and mitochondrial nucleic acids.- 4. Isolation of chloroplasts and chloroplast DNA.- 5. Isolation of total, poly (A) and polysomal RNA from plant tissues.- Section E: Transcription and translation systems.- 1. Assay for gene expression using run-on transcription in isolated nuclei.- 2. Preparation of an in vitro transcription system of plant origin, with methods and templates for assessing its fidelity.- Section F: Blotting and gene detection systems.- 1. Southern, Northern and Western blot analysis.- 2. Screening of cDNA expression libraries with synthetic oligonucleotides for DNA-binding proteins.- 3. Non-radioactive nucleic acid detection systems.- Section G: In situ hybridization and immunodetection.- 1. RNA in situ hybridization in plants.- 2. In situ hybridization to plant metaphase chromosomes using digoxigenin labeled nucleic acid sequences.- Section H: Cloning and detection of DNA sequences from large DNA molecules.- 1. Methods for generating plant genomic libraries Marjory.- 2. Construction of plant yeast artificial chromosome libraries.- 3. Preparation of high molecular weight plant DNA and analysis by pulsed field gel electrophoresis.- 4. Random amplified polymorphic DNA (RAPD) markers.- Section I: Protein-nucleic acid interaction analyses.- 1. Gel mobility shift assay.- 2. Optimization of DNase I footprinting experiments.- 3. Analyses of plant chromatin and in vivo protein-DNA interactions.- 4. Expression and characterization of recombinant plant trans-acting factors.- Section J: Subcellular targeting of proteins.- 1. In vitro import of proteins into chloroplasts.- 2. In vitro targeting of proteins to mitochondria.- 3. Targeting of proteins to the vacuole.- 4. Visualizing protein import into the plant cell nucleus.- Section K: Gene tagging using transposons.- 1. Gene tagging by endogenous transposons.- 2. Heterologous transposon tagging as a tool for the isolation of plant genes. |
| Withdrawn status | Damaged status | Not for loan | Home library | Current library | Date acquired | Source of acquisition | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|
| UE-Central Library | UE-Central Library | 15.07.2019 | U.E.25166 | 581.35 St262 | T10182 | 11.02.2020 | 15.07.2019 | Books |
