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Methods in molecular biology, basic protein and peptide protocols

Walker, M. John

Methods in molecular biology, basic protein and peptide protocols / John M. Walker - Islamabad : NBF, - xii, 490 p. - 32 .

Preface;
Contributors;
the lowry method for protein quantitation;
the bicinchoninic acid assay for protein quantitation;
the braford method for protein quantitation;
nondenaturing Polyacrylamide gel electriohoresis of Proteins;
SDS Polyacrylamide gel electriohoresis of Proteins;
gradient SDS Polyacrylamide gel electriohoresis of Proteins;
Acetic acid-urea Polyacrylamide gel electriohoresis of Proteins;
Electrophoresis of peptides;
Isoelectric focusing of proteins in Ultra-thin Polyacrylamide gels;
The In vivo isotopic labeling of proteins for polyacrylamide gel eltrophoresists;
Structural Profinlings of OLigosaccharides of Glycoproteins;
Drying gels;
Comparison of Protein Primary Structures: peptide Mapping;
Amino Acid Analysis;
Enzymatic methods for cleaving protein;
Chemical Modification of proteins;
The raising of a polyclonal antiserum to a protein;
Enzyme-linked Immunosorbent assay



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