Concept of “Energy from Waste” is now one of the most focused area of work to search solution of energy crisis and manage waste. Among the wastes, fruit wastes are recyclable to be used as a substrate. Being an agricultural country, a bulk of watermelon peels are discarded as waste in Pakistan as well as in other countries due to their excessive use throughout the summer. So to manage this large amount of waste is a huge task that must be done properly in order to save environment from pollution. In Sulphuric acid hydrolysate, the optimum ethanol yield produced by reference yeast Saccharomyces cerevisiae K7 from 52.03 mL hydrolysate at 29.46°C temperature on 15 incubation day was 0.33±0.05 g/g and with Metchnikowia cibodasenis Y34, it was 0.38±0.01 g/g. In hydrochloric acid hydrolysate, the optimum ethanol yield produced with reference yeast S. cerevisiae K7 from 75 mL hydrolysate at 25°C temperature on 15 incubation day was 0.36±0.02 g/g and with Metchnikowia cibodasenis Y34, it was 0.37±0.01 g/g. In nitric acid hydrolysate, the optimum ethanol yield produced with reference yeast S. cerevisiae K7 from 52.03 mL hydrolysate at 29.46°C temperature on 15 incubation day was 0.35±0.07 g/g and with Metchnikowia cibodasenis Y34, it was 0.37±0.03 g/g. The model was significant in case of ethanol yield in all the types of hydrolysate.