This study includes the synthesis, characterization and bio-sccreening of tetra substituted imidazole derivatives. This comprises upon the formation of benzil by the oxidation of benzoin with the concentrated nitric acid. The synthesized benzil was used for the preparation of main product tetra substituted imidazole and its analogues by reacting benzil with ammonium acetate, aniline and different substituted aldehydes in the presence of solvent ethanol maintaining reflux temperature. Verities of tetra substituted imidazole obtained .Structures of these compounds were confirmed by IR and IMS. All prepared compounds were screened for antimicrobial activity against Gram Negative (Escherichia coli, Pseudomonas
eruginosa) and Gram Positive (Bacillus subtilis, staphylococcus aureus) and they showed influential results such as 2-(2,4-dimethoxyphenyl)-1,4,5-triphenyl-1H-imidazole,2-(2,5 dimethoxyphenyl)-1,4,5-triphenyl-1H-imidazole,1,4,5-triphenyl-2-(2,4,5trimethoxyphenyl)-1H-imidazole and 1,2,4,5 tetraphenyl-1H-imidazole show moderate potency against microbes.