000 | 01318nam a22001817a 4500 | ||
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_c21447 _d21447 |
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005 | 20220614151055.0 | ||
008 | 220614b ||||| |||| 00| 0 eng d | ||
020 | _a9780367449476 (pbk) | ||
040 | _cUE-CL | ||
082 |
_a574.8732 _bSh233 |
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100 | _aSharma, Arun Kumar | ||
245 |
_aChromosome techniques _c/ Arun Kumar Sharma & Archana Sharma |
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260 |
_aBoca Raton : _bCRC Press, _c2019 |
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300 | _a368 p. | ||
650 | _aBiology--Chromosome techniques | ||
942 | _cBK | ||
505 | 0 | _a1. Introduction 2. Pre-treatment, hypotonic treatment, fixation and staining 3. Microscopy, micro spectrophotometry flow cytometry, image analysis, confocal microscopy 4. Study of banding patterns of chromosomes 5. Autoradiography light, fibre and high resolution 6. Electron microscopy 7. Study of chromosomes from tissue and protoplast culture, cell fusion and gene transfer in plants 8. Chromosome analysis following short and long-term cultures in mammalian and human systems, from normal and malignant tissues 9. Somatic cell fusion in animals 10. Effects of external agents and monitoring for environmental toxicants 11. Isolation and extraction of nuclei, chromosomes and components 12. Micrurgy 13. Identification of chromosome segments and DNA sequences by in situ molecular hybridization |