000 01318nam a22001817a 4500
999 _c21447
_d21447
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008 220614b ||||| |||| 00| 0 eng d
020 _a9780367449476 (pbk)
040 _cUE-CL
082 _a574.8732
_bSh233
100 _aSharma, Arun Kumar
245 _aChromosome techniques
_c/ Arun Kumar Sharma & Archana Sharma
260 _aBoca Raton :
_bCRC Press,
_c2019
300 _a368 p.
650 _aBiology--Chromosome techniques
942 _cBK
505 0 _a1. Introduction 2. Pre-treatment, hypotonic treatment, fixation and staining 3. Microscopy, micro spectrophotometry flow cytometry, image analysis, confocal microscopy 4. Study of banding patterns of chromosomes 5. Autoradiography light, fibre and high resolution 6. Electron microscopy 7. Study of chromosomes from tissue and protoplast culture, cell fusion and gene transfer in plants 8. Chromosome analysis following short and long-term cultures in mammalian and human systems, from normal and malignant tissues 9. Somatic cell fusion in animals 10. Effects of external agents and monitoring for environmental toxicants 11. Isolation and extraction of nuclei, chromosomes and components 12. Micrurgy 13. Identification of chromosome segments and DNA sequences by in situ molecular hybridization