TY - BOOK AU - Sharma, Arun Kumar TI - Chromosome techniques SN - 9780367449476 (pbk) U1 - 574.8732 PY - 2019/// CY - Boca Raton PB - CRC Press KW - Biology--Chromosome techniques N1 - 1. Introduction 2. Pre-treatment, hypotonic treatment, fixation and staining 3. Microscopy, micro spectrophotometry flow cytometry, image analysis, confocal microscopy 4. Study of banding patterns of chromosomes 5. Autoradiography light, fibre and high resolution 6. Electron microscopy 7. Study of chromosomes from tissue and protoplast culture, cell fusion and gene transfer in plants 8. Chromosome analysis following short and long-term cultures in mammalian and human systems, from normal and malignant tissues 9. Somatic cell fusion in animals 10. Effects of external agents and monitoring for environmental toxicants 11. Isolation and extraction of nuclei, chromosomes and components 12. Micrurgy 13. Identification of chromosome segments and DNA sequences by in situ molecular hybridization ER -